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Pff Series Micro Pore Film Filters

Cell suspensions of 1 ml in 2 ml polypropylene eppendorf vials placed in ice water were lysed by sonication with a micro tip mounted in an mse ultrasonic integrator operated at 21 khz and amplitude setting 3 in six periods of 15 s with 15 s intervals in between.Lysates were centrifuged for 15 min at 16 000g.Supernatants were used for further.

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